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KMID : 0357920000340060395
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Korean Journal of Pathology
2000 Volume.34 No. 6 p.395 ~ p.412
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Gene Expression of Mouse Submandibular Glad during Developmental Stage and Their Antisense Inhibition in Organ Culture
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Kim Yeon-Sook
Lee Suk-Keun
Chi Je-Geun
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Abstract
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This study is aimed to observe the expressions of different genes, including the extracellular matrix proteins, growth factors, and transcription factors during different developmental stages of mouse submandibular gland. Reverse transcription-polymerase chain reaction (RT-PCR) and the antisense inhibition in organ culture system were performed using mouse embryos and newborns. Total 140 mouse embryos (E14(80), E15(20), E16(20), E18(20)) and 30 newborn mice (D2(10), D3(10), D6(10)) obtained from 60 pregnant mice and 3 adult mice (3 weeks old) were used for the cDNA production and the salivary gland organ culture. Syndecan, perlecan, laminin α1 chain, TGF β1, β3, and sonic hedgehog mRNAs were expressed in the early stage (E14∼E16) of the submandibular gland development, whereas transglutaminase C (TGase C), E-cadherin, epimorphin, laminin β2 and γ1 chains, and HGF mRNAs were expressed in the middle and late stages (E16∼E18, D2∼D6). Antisense inhibition of different genes in the organ culture of E14 mouse embryos of submandibular gland showed specific growth retardation in the development of ductal and acinar cells. Especially, the antisense inhibition of perlecan, E-cadherin, laminin α1 chain, laminin β2 chain, and syndecan mRNA arrested the growth of ductal and acinar cells. While the antisense inhibition of integrin β5 greatly affected the acinar cell differentiation and also produced cystic dilatation of salivary ducts, the antisense inhibition of fibronectin showed aberrant growth of ectomesenchymal tissues of the mouse submandibular gland.
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KEYWORD
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Mouse, Submandibular gland, Genes, Organ culture, Antisense inhibition
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